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KMID : 0390020060160040306
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Pediatric Allergy and Respiratory Disease
2006 Volume.16 No. 4 p.306 ~ p.316
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Comparison of Cytokine and Chemokine Production and Activation of NF-κB in RSV-and Adenovirus-infected Bronchial Epithelial Cells
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Cho Kyoung-Soon
Lee Ju-Young
Yoon Jong-Seo
Lee Yoon
Kim Hyun-Hee
Lee Joon-Sung
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Abstract
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Purpose: Our study was designed to evaluate the effects of respiratory syncytial virus (RSV) and adenovirus (Ad) on the production of interleukin (IL)-6, IL-8 and RANTES by BEAS-2B cells. The role of NF-kappaB in the production of those was also studied.
Methoods: We studied the concentration of IL-6, IL-8, RANTES in the culture supernatant of BEAS-2B cell infected by RSV, adenovirus type 3 (Ad3), Ad7. The activation of NF-kappaB was also investigated and the role in the production of IL-6, IL-8, RANTES compared among RSV-, Ad3-, and Ad7- infected cells by electromobility shift assay (EMSA).
Results: The protein production of RANTES, IL-6, and IL-8 in RSV infection was more than Ad infection. The NF-kappaB binding activities in the promotor genes of RANTES, IL-6, IL-8 appeared in RSV, Ad3, and Ad7. These activities of NF-kappaB were inhibited by each gene specific probe.
Conclusion: We suggest that such differences in the immune responses between RSV and Ad may affect the clinical patterns and prognosis after RSV and Ad infection.
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KEYWORD
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Interleukin-6, Interleukin-8, RANTES, NF-κB
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